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avidin biotin peroxidase abc complex  (Vector Laboratories)


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    Vector Laboratories avidin biotin peroxidase abc complex
    Avidin Biotin Peroxidase Abc Complex, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12720 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+biotin+peroxidase+complex/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/bio_rxiv__64898__2026__04__10__717664-207-6-10
    Average 99 stars, based on 12720 article reviews
    avidin biotin peroxidase abc complex - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: Pair bonding sex-specifically improves object recognition memory in monogamous prairie vole.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Olfactory sensory map is perturbed in a human wild-type α-synuclein overexpressing transgenic mouse model of Parkinson’s disease
    Article Snippet: .. After another rinse in PBS, the sections were incubated with avidin-biotin peroxidase complex (cat # PK-6101; Elite ABC-HRP Kit, Vector Laboratories, Inc., Newark, CA) at room temperature for 1 h. The chromogen used was 3-amino-9-ethyl carbazole (AEC Staining Kit, cat # AEC101; Sigma-Aldrich, Inc. St. Louis, MO). .. Slides containing the sections were coverslipped with VectaMount AQ aqueous mounting medium (cat # H-5501-60; Vector Laboratories, Inc.) and images were analyzed with a Keyence BZ-X800 microscope.

    Article Title: Olfactory sensory map is perturbed in a human wild-type α-synuclein overexpressing transgenic mouse model of Parkinson's disease.
    Article Snippet: .. After another rinse in PBS, the sections were incubated with avidin-biotin peroxidase complex (cat # PK-6101; Elite ABC-HRP Kit, Vector Laboratories, Inc., Newark, CA) at room temperature for 1 h. The chromogen used was 3-amino-9-ethyl carbazole (AEC Staining Kit, cat # AEC101; Sigma-Aldrich, Inc. St. Louis, MO). .. Slides containing the sections were coverslipped with VectaMount AQ aqueous mounting medium (cat # H-5501-60; Vector Laboratories, Inc.) and images were analyzed with a Keyence BZ-X800 microscope.

    Article Title: New approach to document cannabis toxicity during pregnancy: a low abundance molecular species detection in long-term formalin-fixed paraffin embedded fetal tissues. A technological report.
    Article Snippet: 1 Faculté de Médecine de Strasbourg, Institut de médecine légale, Université de Strasbourg, 11 rue Humann, Strasbourg 67085, France 2 UF6349 Foetopathologie, Hôpitaux universitaires de Strasbourg, Strasbourg 67000, France 3 Faculté de Médecine de Strasbourg, Institut d’Histologie, Université de Strasbourg, 11 rue Humann, Strasbourg 67000, France 4 X-Pertise Consulting, 42 rue Principale, Mittelhausbergen 67206, France Abstract Background and aims Cannabis is a widely abuse substance during pregnancy.. However, reports of effects on human fetal brain development and implication in brain malformations are scarce in the literature.. This study aims to determine the usability of long-term formalin-fixed tissues, highly degraded as a result of long post-mortem delays, collected during postmortem medical examinations of human fetuses for molecular identification of low-abundance molecular species, namely the cannabinoid receptors, as a prerequisite for studying cannabis role in human fetal brain malformations.

    Article Title: TLR3 activation in astrocytes attenuates the nigrostriatal pathway degeneration in rodent models of Parkinson’s disease
    Article Snippet: Briefly, sections were rinsed in PBS and then incubated with the following primary antibodies: mouse GFAP (Millipore sigma; G3893, 1:500), mouse anti-OX-42 (CD11b; Bio-rad, Hercules, CA, United States; MCA275G, 1:300), mouse anti-TH (Abcam, Cambridge, UK; ab5423, 1:1000), rabbit anti-TH (Pel-freez, Rogers, AR, United States; P40101-150, 1:2000), rabbit anti-TLR3 (Novus Biologicals, Centennial, CO, United States; NB100-56571, 1:1000), rabbit anti-α-synuclein (phospho-S129) (abcam; ab51253, 1:2000), rabbit anti-CNTF (Santa-Cruz Biotechnology, Dallas, TX, United States; sc-13996, 1:200), rabbit anti-VEGF-B (Santa-Cruz Biotechnology; sc-13083, 1:200), and rabbit anti-GDNF (Santa-Cruz Biotechnology; sc-328, 1:400). .. After overnight incubation, in preparation for light microscopy, the brain tissues were incubated with the following biotinylated secondary antibodies: biotin-conjugated anti-mouse (Seracare Life Sciences Inc., Milford, MA, United States; 5260-0051, 1:400), or biotin-conjugated anti-rabbit (Vector Laboratories, Burlingame, CA, United States; BA-1000, 1:400), followed by avidin-biotin-peroxidase complex (Vector Laboratories; PK-6100, 1:1:100). .. The signal was detected by incubating the sections with 3,3′-diaminobenzidine tetrahydrochloride hydrate (DAB; Milliore Sigma; D5637) and viewed under a bright-field microscope (Olympus Optical, Tokyo, Japan).

    Article Title: Increased Tumor Necrosis Factor Superfamily Members in Neuroinflammatory Schizophrenia and Bipolar Disorder Midbrains
    Article Snippet: .. Slides were incubated (4 °C overnight) with primary antibody (mouse anti-FAS, 1:250; sc-8009) washed in 1× phosphate-buffered saline (PBS), and the next day, they were incubated with a secondary antibody (goat anti-mouse IgG biotinylated, 1:500; BA-9200; Vector Laboratories), before being washed and incubated with avidin-biotin-peroxidase complex (PK-4000; VectaStain; Vector Laboratories) followed by PBS washes and visualization with a DAB reaction. ..

    Article Title: Midbrain microglial and macrophage mRNAs distinguish neuroinflammatory schizophrenia from bipolar disorder.
    Article Snippet: Slides were blocked in 10% normal goat serum (S-1000, Vector Laboratories, 237 Burlingame, CA) and incubated (4°C overnight) with primary antibody (mouse anti-CD68 1:400, 238 ab955, Abcam). .. Tissue was washed with phosphate-buffered saline (pH 7.4) and incubated in 239 secondary antibody (goat anti-mouse IgG biotinylated, 1:500, BA-9200, Vector Laboratories) 240 before incubation with avidin-biotin-peroxidase complex (VectaStain, PK-4000, Vector 241 Laboratories) and visualization with a 3,3-diaminobenzidine (DAB) reaction. ..

    Avidin-Biotin Assay:

    Article Title: Pair bonding sex-specifically improves object recognition memory in monogamous prairie vole.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Olfactory sensory map is perturbed in a human wild-type α-synuclein overexpressing transgenic mouse model of Parkinson’s disease
    Article Snippet: .. After another rinse in PBS, the sections were incubated with avidin-biotin peroxidase complex (cat # PK-6101; Elite ABC-HRP Kit, Vector Laboratories, Inc., Newark, CA) at room temperature for 1 h. The chromogen used was 3-amino-9-ethyl carbazole (AEC Staining Kit, cat # AEC101; Sigma-Aldrich, Inc. St. Louis, MO). .. Slides containing the sections were coverslipped with VectaMount AQ aqueous mounting medium (cat # H-5501-60; Vector Laboratories, Inc.) and images were analyzed with a Keyence BZ-X800 microscope.

    Article Title: Olfactory sensory map is perturbed in a human wild-type α-synuclein overexpressing transgenic mouse model of Parkinson's disease.
    Article Snippet: .. After another rinse in PBS, the sections were incubated with avidin-biotin peroxidase complex (cat # PK-6101; Elite ABC-HRP Kit, Vector Laboratories, Inc., Newark, CA) at room temperature for 1 h. The chromogen used was 3-amino-9-ethyl carbazole (AEC Staining Kit, cat # AEC101; Sigma-Aldrich, Inc. St. Louis, MO). .. Slides containing the sections were coverslipped with VectaMount AQ aqueous mounting medium (cat # H-5501-60; Vector Laboratories, Inc.) and images were analyzed with a Keyence BZ-X800 microscope.

    Article Title: New approach to document cannabis toxicity during pregnancy: a low abundance molecular species detection in long-term formalin-fixed paraffin embedded fetal tissues. A technological report.
    Article Snippet: 1 Faculté de Médecine de Strasbourg, Institut de médecine légale, Université de Strasbourg, 11 rue Humann, Strasbourg 67085, France 2 UF6349 Foetopathologie, Hôpitaux universitaires de Strasbourg, Strasbourg 67000, France 3 Faculté de Médecine de Strasbourg, Institut d’Histologie, Université de Strasbourg, 11 rue Humann, Strasbourg 67000, France 4 X-Pertise Consulting, 42 rue Principale, Mittelhausbergen 67206, France Abstract Background and aims Cannabis is a widely abuse substance during pregnancy.. However, reports of effects on human fetal brain development and implication in brain malformations are scarce in the literature.. This study aims to determine the usability of long-term formalin-fixed tissues, highly degraded as a result of long post-mortem delays, collected during postmortem medical examinations of human fetuses for molecular identification of low-abundance molecular species, namely the cannabinoid receptors, as a prerequisite for studying cannabis role in human fetal brain malformations.

    Article Title: TLR3 activation in astrocytes attenuates the nigrostriatal pathway degeneration in rodent models of Parkinson’s disease
    Article Snippet: Briefly, sections were rinsed in PBS and then incubated with the following primary antibodies: mouse GFAP (Millipore sigma; G3893, 1:500), mouse anti-OX-42 (CD11b; Bio-rad, Hercules, CA, United States; MCA275G, 1:300), mouse anti-TH (Abcam, Cambridge, UK; ab5423, 1:1000), rabbit anti-TH (Pel-freez, Rogers, AR, United States; P40101-150, 1:2000), rabbit anti-TLR3 (Novus Biologicals, Centennial, CO, United States; NB100-56571, 1:1000), rabbit anti-α-synuclein (phospho-S129) (abcam; ab51253, 1:2000), rabbit anti-CNTF (Santa-Cruz Biotechnology, Dallas, TX, United States; sc-13996, 1:200), rabbit anti-VEGF-B (Santa-Cruz Biotechnology; sc-13083, 1:200), and rabbit anti-GDNF (Santa-Cruz Biotechnology; sc-328, 1:400). .. After overnight incubation, in preparation for light microscopy, the brain tissues were incubated with the following biotinylated secondary antibodies: biotin-conjugated anti-mouse (Seracare Life Sciences Inc., Milford, MA, United States; 5260-0051, 1:400), or biotin-conjugated anti-rabbit (Vector Laboratories, Burlingame, CA, United States; BA-1000, 1:400), followed by avidin-biotin-peroxidase complex (Vector Laboratories; PK-6100, 1:1:100). .. The signal was detected by incubating the sections with 3,3′-diaminobenzidine tetrahydrochloride hydrate (DAB; Milliore Sigma; D5637) and viewed under a bright-field microscope (Olympus Optical, Tokyo, Japan).

    Article Title: Engram and neural underpinning dynamics of the long-lasting childhood olfactory memory
    Article Snippet: .. To investigate the neural networks activated in response to the childhood odorant, cFos immunochemistry was performed on brain slices (AP coordinates ranging from +6.05 to −2.59) using cFos antibody (1:2000, Abcam, 214672) followed by a biotinylated anti-rabbit secondary antibody (1/200, Vector Laboratories, BA-1000), then revealed through an avidin-biotin-peroxidase complex (ABC Elite Kit 1/100, Vector Laboratories, PK-6100) and amplified with 3,3-Diaminobenzidine (DAB 0.05%, Sigma-Aldrich, D5905). ..

    Article Title: Increased Tumor Necrosis Factor Superfamily Members in Neuroinflammatory Schizophrenia and Bipolar Disorder Midbrains
    Article Snippet: .. Slides were incubated (4 °C overnight) with primary antibody (mouse anti-FAS, 1:250; sc-8009) washed in 1× phosphate-buffered saline (PBS), and the next day, they were incubated with a secondary antibody (goat anti-mouse IgG biotinylated, 1:500; BA-9200; Vector Laboratories), before being washed and incubated with avidin-biotin-peroxidase complex (PK-4000; VectaStain; Vector Laboratories) followed by PBS washes and visualization with a DAB reaction. ..

    Article Title: Midbrain microglial and macrophage mRNAs distinguish neuroinflammatory schizophrenia from bipolar disorder.
    Article Snippet: Slides were blocked in 10% normal goat serum (S-1000, Vector Laboratories, 237 Burlingame, CA) and incubated (4°C overnight) with primary antibody (mouse anti-CD68 1:400, 238 ab955, Abcam). .. Tissue was washed with phosphate-buffered saline (pH 7.4) and incubated in 239 secondary antibody (goat anti-mouse IgG biotinylated, 1:500, BA-9200, Vector Laboratories) 240 before incubation with avidin-biotin-peroxidase complex (VectaStain, PK-4000, Vector 241 Laboratories) and visualization with a 3,3-diaminobenzidine (DAB) reaction. ..

    Staining:

    Article Title: Olfactory sensory map is perturbed in a human wild-type α-synuclein overexpressing transgenic mouse model of Parkinson’s disease
    Article Snippet: .. After another rinse in PBS, the sections were incubated with avidin-biotin peroxidase complex (cat # PK-6101; Elite ABC-HRP Kit, Vector Laboratories, Inc., Newark, CA) at room temperature for 1 h. The chromogen used was 3-amino-9-ethyl carbazole (AEC Staining Kit, cat # AEC101; Sigma-Aldrich, Inc. St. Louis, MO). .. Slides containing the sections were coverslipped with VectaMount AQ aqueous mounting medium (cat # H-5501-60; Vector Laboratories, Inc.) and images were analyzed with a Keyence BZ-X800 microscope.

    Article Title: Olfactory sensory map is perturbed in a human wild-type α-synuclein overexpressing transgenic mouse model of Parkinson's disease.
    Article Snippet: .. After another rinse in PBS, the sections were incubated with avidin-biotin peroxidase complex (cat # PK-6101; Elite ABC-HRP Kit, Vector Laboratories, Inc., Newark, CA) at room temperature for 1 h. The chromogen used was 3-amino-9-ethyl carbazole (AEC Staining Kit, cat # AEC101; Sigma-Aldrich, Inc. St. Louis, MO). .. Slides containing the sections were coverslipped with VectaMount AQ aqueous mounting medium (cat # H-5501-60; Vector Laboratories, Inc.) and images were analyzed with a Keyence BZ-X800 microscope.

    Blocking Assay:

    Article Title: New approach to document cannabis toxicity during pregnancy: a low abundance molecular species detection in long-term formalin-fixed paraffin embedded fetal tissues. A technological report.
    Article Snippet: 1 Faculté de Médecine de Strasbourg, Institut de médecine légale, Université de Strasbourg, 11 rue Humann, Strasbourg 67085, France 2 UF6349 Foetopathologie, Hôpitaux universitaires de Strasbourg, Strasbourg 67000, France 3 Faculté de Médecine de Strasbourg, Institut d’Histologie, Université de Strasbourg, 11 rue Humann, Strasbourg 67000, France 4 X-Pertise Consulting, 42 rue Principale, Mittelhausbergen 67206, France Abstract Background and aims Cannabis is a widely abuse substance during pregnancy.. However, reports of effects on human fetal brain development and implication in brain malformations are scarce in the literature.. This study aims to determine the usability of long-term formalin-fixed tissues, highly degraded as a result of long post-mortem delays, collected during postmortem medical examinations of human fetuses for molecular identification of low-abundance molecular species, namely the cannabinoid receptors, as a prerequisite for studying cannabis role in human fetal brain malformations.

    Light Microscopy:

    Article Title: TLR3 activation in astrocytes attenuates the nigrostriatal pathway degeneration in rodent models of Parkinson’s disease
    Article Snippet: Briefly, sections were rinsed in PBS and then incubated with the following primary antibodies: mouse GFAP (Millipore sigma; G3893, 1:500), mouse anti-OX-42 (CD11b; Bio-rad, Hercules, CA, United States; MCA275G, 1:300), mouse anti-TH (Abcam, Cambridge, UK; ab5423, 1:1000), rabbit anti-TH (Pel-freez, Rogers, AR, United States; P40101-150, 1:2000), rabbit anti-TLR3 (Novus Biologicals, Centennial, CO, United States; NB100-56571, 1:1000), rabbit anti-α-synuclein (phospho-S129) (abcam; ab51253, 1:2000), rabbit anti-CNTF (Santa-Cruz Biotechnology, Dallas, TX, United States; sc-13996, 1:200), rabbit anti-VEGF-B (Santa-Cruz Biotechnology; sc-13083, 1:200), and rabbit anti-GDNF (Santa-Cruz Biotechnology; sc-328, 1:400). .. After overnight incubation, in preparation for light microscopy, the brain tissues were incubated with the following biotinylated secondary antibodies: biotin-conjugated anti-mouse (Seracare Life Sciences Inc., Milford, MA, United States; 5260-0051, 1:400), or biotin-conjugated anti-rabbit (Vector Laboratories, Burlingame, CA, United States; BA-1000, 1:400), followed by avidin-biotin-peroxidase complex (Vector Laboratories; PK-6100, 1:1:100). .. The signal was detected by incubating the sections with 3,3′-diaminobenzidine tetrahydrochloride hydrate (DAB; Milliore Sigma; D5637) and viewed under a bright-field microscope (Olympus Optical, Tokyo, Japan).

    Amplification:

    Article Title: Engram and neural underpinning dynamics of the long-lasting childhood olfactory memory
    Article Snippet: .. To investigate the neural networks activated in response to the childhood odorant, cFos immunochemistry was performed on brain slices (AP coordinates ranging from +6.05 to −2.59) using cFos antibody (1:2000, Abcam, 214672) followed by a biotinylated anti-rabbit secondary antibody (1/200, Vector Laboratories, BA-1000), then revealed through an avidin-biotin-peroxidase complex (ABC Elite Kit 1/100, Vector Laboratories, PK-6100) and amplified with 3,3-Diaminobenzidine (DAB 0.05%, Sigma-Aldrich, D5905). ..

    Saline:

    Article Title: Increased Tumor Necrosis Factor Superfamily Members in Neuroinflammatory Schizophrenia and Bipolar Disorder Midbrains
    Article Snippet: .. Slides were incubated (4 °C overnight) with primary antibody (mouse anti-FAS, 1:250; sc-8009) washed in 1× phosphate-buffered saline (PBS), and the next day, they were incubated with a secondary antibody (goat anti-mouse IgG biotinylated, 1:500; BA-9200; Vector Laboratories), before being washed and incubated with avidin-biotin-peroxidase complex (PK-4000; VectaStain; Vector Laboratories) followed by PBS washes and visualization with a DAB reaction. ..

    Article Title: Midbrain microglial and macrophage mRNAs distinguish neuroinflammatory schizophrenia from bipolar disorder.
    Article Snippet: Slides were blocked in 10% normal goat serum (S-1000, Vector Laboratories, 237 Burlingame, CA) and incubated (4°C overnight) with primary antibody (mouse anti-CD68 1:400, 238 ab955, Abcam). .. Tissue was washed with phosphate-buffered saline (pH 7.4) and incubated in 239 secondary antibody (goat anti-mouse IgG biotinylated, 1:500, BA-9200, Vector Laboratories) 240 before incubation with avidin-biotin-peroxidase complex (VectaStain, PK-4000, Vector 241 Laboratories) and visualization with a 3,3-diaminobenzidine (DAB) reaction. ..



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